Anti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband®, PE Conjugate

About this product

Boster Bio Anti-Mitochondrial dicarboxylate carrier/SLC25A10 Antibody Picoband® catalog # A10727-3. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A10727-3-PE

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

The mitochondrial dicarboxylate carrier (DIC) is an integral membrane protein encoded by the SLC25A10 gene in humans that catalyzes the transport of dicarboxylates such as malonate, malate, and succinate across the inner mitochondrial membrane in exchange for phosphate, sulfate, and thiosulfate by a simultaneous antiport mechanism, thus supplying substrates for the Krebs cycle, gluconeogenesis, urea synthesis, fatty acid synthesis, and sulfur metabolism. This gene encodes a member of a family of proteins that translocate small metabolites across the mitochondrial membrane. The encoded protein exchanges dicarboxylates, such as malate and succinate, for phosphate, sulfate, and other small molecules, thereby providing substrates for metabolic processes including the Krebs cycle and fatty acid synthesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Synonyms

RNA-binding protein Nova-2; Astrocytic NOVA1-like RNA-binding protein; Neuro-oncological ventral antigen 2; NOVA2; ANOVA; NOVA3

Gene Name

NOVA alternative splicing regulator 2

Gene ID

1468

UniProt

Q9UBX3

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Mitochondrial dicarboxylate carrier/SLC25A10 recombinant protein (Position: Q37-Q276).

Clonality

Polyclonal

Tissue Specificity

Brain. Expression restricted to astrocytes.

Applications

WB,IHC,IF,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

May regulate RNA splicing or metabolism in a specific subset of developing neurons. Binds single strand RNA.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Fiermonte, G., Dolce, V., Arrigoni, R., Runswick, M. J., Walker, J. E., Palmieri, F. Organization and sequence of the gene for the human mitochondrial dicarboxylate carrier: evolution of the carrier family. Biochem. J. 344: 953-960, 1999. 2. Mizuarai, S., Miki, S., Araki, H., Takahashi, K., Kotani, H. Identification of dicarboxylate carrier Slc25a10 as malate transporter in de novo fatty acid synthesis. J. Biol. Chem. 280: 32434-32441, 2005. 3. Pannone, E., Fiermonte, G., Dolce, V., Rocchi, M., Palmieri, F. Assignment of the human dicarboxylate carrier gene (DIC) to chromosome 17 band 17q25.3. Cytogenet. Cell Genet. 83: 238-239, 1998.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

29370 MW

Observed Molecular Weight

29-31 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat<br> Immunofluorescence, 5 μg/ml, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Nucleus.

Protein Name

Aquaporin-6