Anti-VRL1/TRPV2 Antibody Picoband®, PE Conjugate

About this product

Boster Bio Anti-VRL1/TRPV2 Antibody catalog # PA1977. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1977-PE

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

TRPV2 (Transient Receptor Potential Cation Channel Subfamily V Member 2), also known as VRL1, is a protein that, in humans, is encoded by the TRPV1 gene. The International Radiation Hybrid Mapping Consortium mapped the TRPV2 gene to chromosome 17. This gene encodes an ion channel that is activated by high temperatures above 52??C. The protein may be involved in transduction of high-temperature heat responses in sensory ganglia. It is though that in other tissues the channel may be activated by stimuli other than heat.

Synonyms

Transient receptor potential cation channel subfamily V member 2;TrpV2;Osm-9-like TRP channel 2;OTRPC2;Vanilloid receptor-like protein 1;VRL-1;TRPV2;VRL;

Gene Name

Transient receptor potential cation channel subfamily V member 2

UniProt

Q9Y5S1

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human VRL1.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells.

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by IGF-I, PDGF and neuropeptide head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH. .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Caterina, M. J., Rosen, T. A., Tominaga, M., Brake, A. J., Julius, D. A capsaicin-receptor homologue with a high threshold for noxious heat. Nature 398: 436-441, 1999. 2. Iwata, Y., Katanosaka, Y., Arai, Y., Shigekawa, M., Wakabayashi, S. Dominant-negative inhibition of Ca(2+) influx via TRPV2 ameliorates muscular dystrophy in animal models. Hum. Molec. Genet. 18: 824-834, 2009.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

85981 MW

Observed Molecular Weight

450 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -<br>Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat<br>Western blot, 0.1-0.5μg/ml, Human<br>

Subcellular Location

Cell membrane ; Multi-pass membrane protein . Cytoplasm . Melanosome . Translocates from the cytoplasm to the plasma membrane upon ligand stimulation (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .

Sequence Similarities

Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV2 sub-subfamily.

Protein Name

Transient receptor potential cation channel subfamily V member 2