Anti-LRP5 Antibody Picoband®, PE Conjugate

About this product

Boster Bio Anti-LRP5 Antibody catalog # PA2273. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2273-PE

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Low-density lipoprotein receptor-related protein 5 is a protein that in humans is encoded by the LRP5 gene. It is mapped to 11q13.2. LRP5 is a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with diabetes mellitus type 1 in humans. This protein plays a key role in skeletal homeostasis. In addition to that, the binding of axin to LRP5 is an important part of the Wnt signal transduction pathway, and it also acts as a target for the inhibitory effects of Dickkopf, another developmental protein, on Wnt signaling.

Synonyms

Low-density lipoprotein receptor-related protein 5;LRP-5;LRP5;LR3, LRP7;

Gene Name

Low-density lipoprotein receptor-related protein 5

UniProt

O75197

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human LRP5, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Widely expressed, with the highest level of expression in the liver and in aorta. .

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Appears be required for postnatal control of vascular regression in the eye. Required for posterior patterning of the epiblast during gastrulation. .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Hey PJ, Twells RC, Phillips MS, Yusuke Nakagawa, Brown SD, Kawaguchi Y, Cox R, Guochun Xie, Dugan V, Hammond H, Metzker ML, Todd JA, Hess JF (Oct 1998). "Cloning of a novel member of the low-absorbance lipoprotein receptor family". Gene 216 (1): 103–11. 2. "Entrez Gene: LRP5 low absorbance lipoprotein receptor-related protein 5" 3. Mao, J., Wang, J., Liu, B., Pan, W., Farr, G. H., III, Flynn, C., Yuan, H., Takada, S., Kimelman, D., Li, L., Wu, D. Low-absorbance lipoprotein receptor-related protein-5 binds to axin and regulates the canonical Wnt signaling pathway. Molec. Cell 7: 801-809, 2001.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

179145 MW

Observed Molecular Weight

179 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human<br>

Subcellular Location

Membrane; Single-pass type I membrane protein. Endoplasmic reticulum . Chaperoned to the plasma membrane by MESD. .

Sequence Similarities

Belongs to the LDLR family.

Protein Name

Low-density lipoprotein receptor-related protein 5