Anti-MIF Antibody Picoband® (HRP Conjugate)

About this product

Boster Bio Anti-MIF Antibody Picoband® catalog # PB9274. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9274-HRP

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Macrophage migration inhibitory factor (MIF or MMIF), also known as GIF, is a protein that in humans is encoded by the MIF gene. It is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. The localization of the human gene for MIF is to chromosome 22q11.2. MIF plays a critical role in inflammatory diseases and atherogenesis. It is also involved in cell-mediated immunity and immunoregulation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids.

Synonyms

Macrophage migration inhibitory factor;MIF;5.3.2.1;Glycosylation-inhibiting factor;GIF;L-dopachrome isomerase;L-dopachrome tautomerase;5.3.3.12;Phenylpyruvate tautomerase;MIF;GLIF, MMIF;

Gene Name

Macrophage migration inhibitory factor

UniProt

P14174

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human MIF recombinant protein (Position: P2-A115). Human MIF shares 89% and 90% amino acid (aa) sequence identity with mouse and rat MIF respectively.

Clonality

Polyclonal

Tissue Specificity

Plasma. Expressed in liver. .

Applications

IF,IHC,ICC,WB,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti- inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human<br>

Subcellular Location

Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non- classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.

Sequence Similarities

Belongs to the MIF family.

Protein Name

Macrophage migration inhibitory factor