Anti-Lipocalin 2/LCN2 Antibody Picoband® (HRP Conjugate)

About this product

Boster Bio <a href="https://www.bosterbio.com/anti-lipocalin-2-ngal-picoband-trade-antibody-a00452-boster.html" style="color:#EA8D28">Anti-Lipocalin 2</a>/LCN2 Antibody Picoband&reg; catalog # PB9609. Tested in ELISA, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9609-HRP

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.

Synonyms

Neutrophil gelatinase-associated lipocalin;NGAL;Alpha-2-microglobulin-related protein;Alpha-2U globulin-related protein;Lipocalin-2;Siderocalin LCN2;p25;Lcn2;

Gene Name

Neutrophil gelatinase-associated lipocalin

UniProt

P30152

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived rat Lipocalin 2 recombinant protein (Position: Q21-N198). Rat Lipocalin 2 shares 64.4 % and 81.1 % amino acid (aa) sequence identity with human and mouse Lipocalin 2, respectively.

Clonality

Polyclonal

Tissue Specificity

Detected in lung, spleen, uterus, vagina and epididymis. .

Applications

ELISA,IHC,IHC-F,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5- DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth (By similarity). .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Mouse<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat<br> Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat<br> ELISA (cap) ,1-5μg/ml, Rat<br>

Subcellular Location

Secreted . Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. .

Sequence Similarities

Belongs to the KIN17 family.

Protein Name

Neutrophil gelatinase-associated lipocalin