Anti-SMURF1 Antibody Picoband® (HRP Conjugate)

About this product

Boster Bio Anti-SMURF1 Antibody Picoband® catalog # PB9892. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9892-HRP

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

E3 ubiquitin-protein ligase SMURF1 is an enzyme that in humans is encoded by the SMURF1 gene. It is mapped to 7q22.1. This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms

E3 ubiquitin-protein ligase SMURF1;hSMURF1;6.3.2.-;SMAD ubiquitination regulatory factor 1;SMAD-specific E3 ubiquitin-protein ligase 1;SMURF1;KIAA1625;

Gene Name

E3 ubiquitin-protein ligase SMURF1

UniProt

Q9HCE7

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SMURF1, identical to the related mouse sequence.

Clonality

Polyclonal

Tissue Specificity

Expressed in melanocytes (PubMed:23999003). .

Applications

Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Plays a role in dendrite formation by melanocytes (PubMed:23999003). .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat<br> Immunocytochemistry/Immunofluorescence, 2μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human<br>

Subcellular Location

Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side .

Protein Name

E3 ubiquitin-protein ligase SMURF1