Anti-MED9 Antibody Picoband® (HRP Conjugate)

About this product

Boster Bio Anti-MED9 Antibody Picoband® catalog # A12781. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A12781-HRP

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

MED9 is a member of the mediator complex of proteins that promote activation of RNA polymerase II through interactions with transcription factors. This MED9gene is located within the Smith-Magenis syndrome region on chromosome 17.

Synonyms

Mediator of RNA polymerase II transcription subunit 9; Mediator complex subunit 9; MED9; MED25;

Gene Name

mediator complex subunit 9

UniProt

MED9: Q9NWA0

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human MED9 recombinant protein (Position: A55-E146).

Clonality

Polyclonal

Tissue Specificity

Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Applications

ELISA,Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml<br> Immunocytochemistry/Immunofluorescence, 2μg/ml<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells<br> ELISA, 0.1-0.5μg/ml<br>

Subcellular Location

Nucleus .