Anti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7) (HRP Conjugate)

About this product

Boster Bio Anti-TCP1 alpha Antibody Picoband® (monoclonal, 2E7) catalog # M02389. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M02389-HRP

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

T-complex protein 1 subunit alpha is a protein that in humans is encoded by the TCP1 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found.

Synonyms

T-complex protein 1 subunit alpha; TCP-1-alpha; CCT-alpha; TCP1; CCT1; CCTA

Gene Name

t-complex 1

UniProt

P17987

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TCP1 alpha, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Clonality

Monoclonal

Clone

Clone: 2E7

Tissue Specificity

Widely expressed. Highest level in placenta, liver, muscle and kidney.

Applications

Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Mouse IgG1

Applications Notes

Western blot, 0.1-0.5μg/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml<br> Immunocytochemistry/Immunofluorescence, 2μg/ml<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells

Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Protein Name

T-complex protein 1 subunit alpha