Anti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband®, HRP Conjugate

About this product

Boster Bio Anti-Troponin I fast skeletal muscle/TNNI2 Antibody Picoband® catalog # A07355-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A07355-2-HRP

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Troponin I, fast skeletal muscle is a protein that in humans is encoded by the TNNI2 gene. It is mapped to 11p15.5. This gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible for the calcium-dependent regulation of striated muscle contraction. Mouse studies show that this component is also present in vascular smooth muscle and may play a role in regulation of smooth muscle function. In addition to muscle tissues, this protein is found in corneal epithelium, cartilage where it is an inhibitor of angiogenesis to inhibit tumor growth and metastasis, and mammary gland where it functions as a co-activator of estrogen receptor-related receptor alpha. This protein also suppresses tumor growth in human ovarian carcinoma. Mutations in this gene cause myopathy and distal arthrogryposis type 2B. Alternatively spliced transcript variants have been found for this gene.

Synonyms

Troponin I, fast skeletal muscle; Troponin I, fast-twitch isoform; TNNI2

Gene Name

troponin I2, fast skeletal type

Gene ID

7136

UniProt

P48788

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Troponin I fast skeletal muscle/TNNI2 recombinant protein (Position: D3-S182).

Clonality

Polyclonal

Tissue Specificity

Highly expressed in brain and weakly in heart, small intestine and uterus. Isoform 1A is mostly expressed in granular cell and molecular layer. Isoform 1B is mostly expressed in Purkinje cells. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland. .

Applications

ELISA,IF,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

References & Citations

1. Barton, P. J. R., Townsend, P. J., Brand, N. J., Yacoub, M. H. Localization of the fast skeletal muscle troponin I gene (TNNI2) to 11p15.5: genes for troponin I and T are organized in pairs. Ann. Hum. Genet. 61: 519-523, 1997. 2. Drera, B., Zoppi, N., Barlati, S., Colombi, M. Recurrence of the p.R156X TNNI2 mutation in distal arthrogryposis type 2B. (Letter) Clin. Genet. 70: 532-534, 2006.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

108320 MW

Observed Molecular Weight

24 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.25μg/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml<br> Immunofluorescence, 5 μg/ml<br> ELISA, 0.1-0.5μg/ml<br>

Subcellular Location

Troponin complex. Cytosol. Nucleus.

Protein Name

Gamma-aminobutyric acid type B receptor subunit 1