Anti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4), HRP Conjugate

About this product

Boster Bio Anti-MPPB/PMPCB Antibody Picoband® (monoclonal, 9F13E4) catalog # M11793. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M11793-HRP

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mitochondrial-processing peptidase subunit beta is an enzyme that in humans is encoded by the PMPCB gene. This gene is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.

Synonyms

ATP synthase D chain mitochondrial antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody|ATP synthase subunit d antibody|ATP synthase subunit d, mitochondrial antibody|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody|ATP5H antibody|ATP5H_HUMAN antibody|ATP5JD antibody|ATPase subunit d antibody|ATPQ antibody|mitochondrial antibody|My032 protein antibody

Gene Name

ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d

Gene ID

9512

UniProt

O75439

Host

Mouse

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human MPPB/PMPCB recombinant protein (Position: E23-Q479).

Clonality

Monoclonal

Clone

Clone: 9F13E4

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Applications

WB,IHC,ICC,IF,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Mitochondrial membrane ATP synthase (F (1)F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F (0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha (3)beta (3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

References & Citations

1. Fields, S., Song, O. A novel genetic system to detect protein-protein interactions. (Letter) Nature 340: 245-246, 1989. 2. Gordon, D. M., Shi, Q., Dancis, A., Pain, D. Maturation of frataxin within mammalian and yeast mitochondria: one-step processing by matrix processing peptidase. Hum. Molec. Genet. 8: 2255-2262, 1999. 3. Gross, M. B. Personal Communication. Baltimore, Md. 5/3/2018.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

43 kDa

Fragment

Mouse IgG2b

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human<br>

Subcellular Location

Mitochondrion. Mitochondrion inner membrane.

Protein Name

ATP synthase subunit d, mitochondrial