Anti-Golgin 97/GOLGA1 Antibody Picoband® (monoclonal, 8E4H1), HRP Conjugate

About this product

Boster Bio Anti-Golgin 97/GOLGA1 Antibody Picoband® (monoclonal, 8E4H1) catalog # M13524-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M13524-1-HRP

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.

Synonyms

ATP synthase D chain mitochondrial antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody|ATP synthase subunit d antibody|ATP synthase subunit d, mitochondrial antibody|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody|ATP5H antibody|ATP5H_HUMAN antibody|ATP5JD antibody|ATPase subunit d antibody|ATPQ antibody|mitochondrial antibody|My032 protein antibody

Gene Name

ATP synthase, H+ transporting, mitochondrial Fo complex, subunit d

Gene ID

2800

UniProt

Q92805

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human Golgin 97/GOLGA1 recombinant protein (Position: M1-K752).

Clonality

Monoclonal

Clone

Clone: 8E4H1

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Applications

WB,ICC,IF,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Mitochondrial membrane ATP synthase (F (1)F (0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F (1) - containing the extramembraneous catalytic core, and F (0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F (1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F (0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha (3)beta (3) subcomplex and subunit a/ATP6 static relative to the rotary elements.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

References & Citations

1. Griffith, K. J., Chan, E. K. L., Lung, C.-C., Hamel, J. C., Guo, X., Miyachi, K., Fritzler, M. J. Molecular cloning of a novel 97-Kd Golgi complex autoantigen associated with Sjogren's syndrome. Arthritis Rheum. 40: 1693-1702, 1997. 2. Lu, L., Tai, G., Hong, W. Autoantigen golgin-97, an effector of Arl1 GTPase, participates in traffic from the endosome to the trans-Golgi network. Molec. Biol. Cell 15: 4426-4443, 2004. 3. Wong, M., Munro, S. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins. Science 346: 1256898, 2014. Note: Electronic Article.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

97 kDa

Fragment

IgG1

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human<br>

Subcellular Location

Mitochondrion. Mitochondrion inner membrane.

Protein Name

ATP synthase subunit d, mitochondrial