Anti-OGFOD3 Antibody Picoband®, HRP Conjugate

About this product

Boster Bio Anti-OGFOD3 Antibody Picoband® catalog # A15361-1. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A15361-1-HRP

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

OGFOD3 (2-oxoglutarate and iron-dependent oxygenase domain-containing protein 3) is a protein involved in the hydroxylation of transfer RNA (tRNA), specifically targeting tRNA-hypoxanthine modification. This process is crucial for maintaining accurate translation and protein synthesis in cells. OGFOD3 belongs to the family of 2-oxoglutarate (2OG)-dependent oxygenases, which require iron and 2OG as cofactors to catalyze various oxidative reactions. Its role highlights the importance of tRNA modifications in cellular physiology, potentially influencing gene expression and protein function regulation.

Gene Name

OGFOD3

Gene ID

79701

UniProt

Q6PK18

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

E.coli-derived human OGFOD3 recombinant protein (Position: D67-H311). Human OGFOD3 shares 92.2% and 90.6% amino acid (aa) sequence identity with mouse and rat OGFOD3, respectively.

Clonality

Polyclonal

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Wang, S., Singh, S. K., Katika, M. R., Lopez-Aviles, S., & Hurtado, A. (2018). High throughput chemical screening reveals multiple regulatory proteins on FOXA1 in breast cancer cell lines. International Journal of Molecular Sciences, 19(12), 4123. 2. Maio, N., Kim, K. S., Singh, A., & Rouault, T. A. (2017). A single adaptable cochaperone-scaffold complex delivers nascent iron-sulfur clusters to mammalian respiratory chain complexes I–III. Cell metabolism, 25(4), 945-953. 3. Liu, X., Salokas, K., Tamene, F., Jiu, Y., Weldatsadik, R. G., Öhman, T., & Varjosalo, M. (2018). An AP-MS-and BioID-compatible MAC-tag enables comprehensive mapping of protein interactions and subcellular localizations. Nature communications, 9(1), 1188.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Applications Notes

Western blot, 0.25-0.5 μg/ml, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>