- Catalog number
- PA1245-1-HRP
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cyclin D1, also known as CCND1, is a human gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclin D1 encodes the regulatory subunit of a holoenzyme that phosphorylates and inactivates the retinoblastoma protein and promotes progression through the G1-S phase of the cell cycle. Amplification or overexpression of cyclin D1 plays pivotal roles in the development of a subset of human cancers including parathyroid adenoma, breast cancer, colon cancer, lymphoma, melanoma, and prostate cancer. The cyclin D1 gene is overexpressed in human breast cancers and is required for oncogene-induced tumorigenesis. Brisken et al. (2003) found that prolactin (PRL; 176760) induced IGF2 (147470) mRNA and IGF2 induced cyclin D1 protein expression in mouse mammary epithelial cultures. And they also concluded that IGF2 is a mediator of prolactin-induced alveologenesis and that prolactin, IGF2, and cyclin D1 are components of a developmental pathway in mammary gland.
- Synonyms
- G1/S-specific cyclin-D1;Ccnd1;Cyl-1;
- Gene Name
- G1/S-specific cyclin-D1
- UniProt
- P25322
- Host
- Rabbit
- Reactivity
- Human,Mouse,Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of mouse Cyclin D1, identical to the related rat sequence, and different from the related human sequence by three amino acids.
- Clonality
- Polyclonal
- Applications
- WB
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity). .
- Components
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
- References & Citations
- 1. Fu, M.; Wang, C.; Li, Z.; Sakamaki, T.; Pestell, R. G. : Minireview: Cyclin D1: normal and abnormal functions. Endocrinology 145: 5439-5447, 2004.
2. Wang, C.; Pattabiraman, N.; Zhou, J. N.; Fu, M.; Sakamaki, T.; Albanese, C.; Li, Z.; Wu, K.; Hulit, J.; Neumeister, P.; Novikoff, P. M.; Brownlee, M.; Scherer, P. E.; Jones, J. G.; Whitney, K. D.; Donehower, L. A.; Harris, E. L.; Rohan, T.; Johns, D. C.; Pestell, R. G. : Cyclin D1 repression of peroxisome proliferator-activated receptor gamma expression and transactivation. Molec. Cell. Biol. 23: 6159-6173, 2003.
3. Brisken, C.; Ayyannan, A.; Nguyen, C.; Heineman, A.; Reinhardt, F.; Tan, J.; Dey, S. K.; Dotto, G. P.; Weinberg, R. A. : IGF-2 is a mediator of prolactin-induced morphogenesis in the breast. Dev. Cell 3: 877-887, 2002. Note: Erratum: Dev. Cell 4: 283 only, 2003.
- Storage Conditions
- Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 33429 MW
- Observed Molecular Weight
- 54 kDa
- Fragment
- Rabbit IgG
- Specificity
- No cross reactivity with other proteins.
- Applications Notes
- Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse<br>
- Subcellular Location
- Nucleus . Cytoplasm . Membrane . Cyclin D- CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.
- Sequence Similarities
- Belongs to the cyclin family. Cyclin D subfamily.
- Protein Name
- G1/S-specific cyclin-D1
