Anti-Superoxide Dismutase 1/SOD1 Antibody Picoband®, HRP Conjugate

About this product

Boster Bio Anti-Superoxide Dismutase 1/SOD1 Antibody catalog # PA1345. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1345-HRP

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Superoxide dismutases (SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. One of the exceedingly rare exceptions is Lactobacillus plantarum and related lactobacilli, which use a different mechanism.Cu,Zn-SOD was found widely distributed in the cell cytosol and in the cell nucleus, consistent with it being a soluble cytosolic protein. Mitochondria and secretory compartments did not label for this protein. In human cells, peroxisomes showed a labeling density slightly less than that of cytoplasm.

Synonyms

Superoxide dismutase [Cu-Zn];1.15.1.1;Superoxide dismutase 1;hSod1;SOD1;

Gene Name

Superoxide dismutase [Cu-Zn]

UniProt

P00441

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of Human SOD1, different from the mouse sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.

Applications

IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Crapo, J. D., Oury, T., Rabouille, C., Slot, J. W., Chang, L.-Y. Copper,zinc superoxide dismutase is primarily a cytosolic protein in human cells. Proc. Nat. Acad. Sci. 89: 10405-10409, 1992.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

15936 MW

Observed Molecular Weight

18 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat <br>Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat<br>Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, Human<br>Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br>

Subcellular Location

Cytoplasm. Nucleus. Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.

Sequence Similarities

Belongs to the Cu-Zn superoxide dismutase family.

Protein Name

Superoxide dismutase[Cu-Zn]