Anti-Kallikrein 1/KLK1 Antibody Picoband®, HRP Conjugate

About this product

Boster Bio Anti-Kallikrein 1/KLK1 Antibody catalog # PA1625. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1625-HRP

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

KLK1 (KALLIKREIN 1), also called KLKR, is a protein that in humans is encoded by the KLK1 gene. KLK1 is a member of the peptidase S1 family. KLK1 is a serine protease that generates Lys-bradykinin by specific proteolysis of kininogen-1. The KLK1 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19 and its exact cytogenetic location is 19q13.33. The KLK1 gene contains 5 coding exons. And KLK1 is the most centromeric gene in the cluster. Mice lacking tissue kallikrein are unable to generate significant levels of kinins in most tissues and develop cardiovascular abnormalities early in adulthood despite normal blood pressure. The protein is functionally conserved in its capacity to release the vasoactive peptide, Lys-bradykinin, from low molecular weight kininogen.

Synonyms

Kallikrein-1;3.4.21.35;Kidney/pancreas/salivary gland kallikrein;Tissue kallikrein;KLK1;

Gene Name

Kallikrein-1

UniProt

P06870

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Kallikrein 1.

Clonality

Polyclonal

Tissue Specificity

Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. .

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Azizi, M., Boutouyrie, P., Bissery, A., Agharazii, M., Verbeke, F., Stern, N., Bura-Riviere, A., Laurent, S., Alhenc-Gelas, F., Jeunemaitre, X. Arterial and renal consequences of partial genetic deficiency in tissue kallikrein activity in humans. J. Clin. Invest. 115: 780-787, 2005. 2. Baker, A. R., Shine, J. Human kidney kallikrein: cDNA cloning and sequence analysis. DNA 4: 445-450, 1985. 3. Clements J, Hooper J, Dong Y, Harvey T (2001). "The expanded human kallikrein (KLK) gene family: genomic organisation, tissue-specific expression and potential functions".

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

28890 MW

Observed Molecular Weight

29 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat<br>

Subcellular Location

Membrane ; Single-pass type I membrane protein .

Sequence Similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein Name

Kallikrein-1