Anti-Cpn10/HSPE1 Antibody Picoband®, HRP Conjugate

About this product

Boster Bio Anti-Cpn10/HSPE1 Antibody catalog # PA1790. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1790-HRP

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

HSPE1 (heat shock 10kDa protein 1 (chaperonin 10)), also called CPN10, GROES, CHAPERONIN 10 HOMOLOG, cpn10 HOMOLOG or HSP10, is a protein that in humans is encoded by the HSPE1 gene. GroES is a heptameric ring of identical 10.4-kD subunits that binds to each end of GroEL to form a symmetric, functional heterodimer. The HSP10 gene consists of 4 exons. The HSPE1 gene is mapped to 2q33.1. The transcriptional activity of the promoter fragment in the HSP60 ion is approximately twice that in the HSP10 ion under normal growth conditions; upon heat shock, promoter activity in either ion increased by a factor of approximately 12. Mutational drifts performed in vitro with 4 different enzymes indicated the GroES overexpression doubled the number of accumulating mutations, and promoted the folding of enzyme variants carrying mutations in the protein core and/or mutations with higher destabilizing effects.

Synonyms

10 kDa heat shock protein, mitochondrial;Hsp10;10 kDa chaperonin;Chaperonin 10;CPN10;Early-pregnancy factor;EPF;HSPE1;

Gene Name

10 kDa heat shock protein, mitochondrial

UniProt

P61604

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Cpn10, different from the related rat and mouse sequences by one amino acid.

Clonality

Polyclonal

Applications

Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Georgopoulos, C., Welch, W. J. Role of the major heat shock proteins as molecular chaperones. Annu. Rev. Cell Biol. 9: 601-634, 1993. 2. Hansen, J. J., Bross, P., Westergaard, M., Nielsen, M. N., Eiberg, H., Borglum, A. D., Mogensen, J., Kristiansen, K., Bolund, L., Gregersen, N. Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a biional promoter. Hum. Genet. 112: 71-77, 2003. Note: Erratum: Hum. Genet. 112: 436 only, 2003. 3. Todd, M. J., Viitanen, P. V., Lorimer, G. H. Dynamics of the chaperonin ATPase cycle: implications for facilitated protein folding. Science 265: 659-666, 1994.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

10932 MW

Observed Molecular Weight

11 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat<br> Immunocytochemistry , 0.5-1μg/ml, Human<br> Immunofluorescence, 2μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human<br>

Subcellular Location

Mitochondrion matrix.

Sequence Similarities

Belongs to the heat shock protein 70 family.

Protein Name

10 kDa heat shock protein, mitochondrial(Hsp10)