Anti-SIRT3 Antibody Picoband® (APC Conjugate)

About this product

Boster Bio Anti-SIRT3 Antibody Picoband® catalog # PB9161. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9161-APC

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is a protein that in humans is encoded by the SIRT3 gene. SIRT3 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. It is mapped to 11p15.5. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of SIRT3 in cultured cells increases respiration and decreases the production of reactive oxygen species. SIRT3 can function as a mitochondrial deacetylase. In addition to it, human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.

Synonyms

NAD-dependent protein deacetylase sirtuin-3, mitochondrial;hSIRT3;3.5.1.-;Regulatory protein SIR2 homolog 3;SIR2-like protein 3;SIRT3;SIR2L3;

Gene Name

NAD-dependent protein deacetylase sirtuin-3, mitochondrial

UniProt

Q9NTG7

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human SIRT3 recombinant protein (Position: P66-K399). Human SIRT3 shares 86% amino acid (aa) sequence identity with mouse SIRT3.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. .

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Rat<br>

Subcellular Location

Mitochondrion matrix .

Sequence Similarities

Belongs to the sirtuin family. Class I subfamily.

Protein Name

NAD-dependent protein deacetylase sirtuin-3, mitochondrial