Anti-NUP98 Antibody Picoband® (APC Conjugate)

About this product

Boster Bio Anti-NUP98 Antibody Picoband® catalog # PB9302. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9302-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nuclear pore complex protein Nup98-Nup96 is a protein that in humans is encoded by the NUP98 gene. This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. NUP98 is a peripheral nucleoporin located at both the cytoplasmic and nuclear sides of the central channel of the NPC. NUP98 phosphorylation is critical for NPC disassembly at the onset of mitosis. It also plays roles in gene expression, mitotic checkpoint, and pathogenesis. Ligand blot analysis suggested that NUP98 can function as a docking protein for cytosol-mediated docking of import substrates. In addition to that, NUP98 is a target of the vesicular stomatitis virus M protein-mediated inhibition of mRNA nuclear export.

Synonyms

Nuclear pore complex protein Nup98-Nup96;Nuclear pore complex protein Nup98;98 kDa nucleoporin;Nucleoporin Nup98;Nup98;Nuclear pore complex protein Nup96;96 kDa nucleoporin;Nucleoporin Nup96;Nup96;NUP98;ADAR2;

Gene Name

Nuclear pore complex protein Nup98-Nup96

UniProt

P52948

Host

Rabbit

Reactivity

Human,Monkey,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human NUP98 recombinant protein (Position: H549-F880). Human NUP98 shares 95% and 94% amino acid (aa) sequence identity with mouse and rat NUP98, respectively.

Clonality

Polyclonal

Tissue Specificity

The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Nup98 and Nup96 are involved in the biional transport across the NPC. May anchor NUP153 and TPR to the NPC. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat<br>

Subcellular Location

Nucleus. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Nucleus, nuclear pore complex. Nup96 is localized to the nucleoplasmic side of the nuclear pore complex (NPC), at or near the nucleoplasmic basket. Dissociates from the dissasembled NPC structure early during prophase of mitosis. Colocalized with NUP153 and TPR to the nuclear basket of NPC. Detected in diffuse and discrete intranuclear foci. Remained localized to the nuclear membrane after poliovirus (PV) infection.

Sequence Similarities

Belongs to the nucleoporin GLFG family.

Protein Name

Nuclear pore complex protein Nup98-Nup96