Anti-HEF1/NEDD9 Antibody Picoband® (APC Conjugate)

About this product

Boster Bio Anti-HEF1/NEDD9 Antibody Picoband® catalog # PB9289. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9289-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Enhancer of filamentation 1 (EF1), also known as NEDD-9, is a protein that in humans is encoded by the NEDD9 gene. The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. It is mapped to 6p24.2. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. NEDD9 is also a highly relevant cancer gene that governs metastatic potential in human melanoma. What’s more, it has been speculated that NEDD9 may be an important linking element between extracellular signaling and regulation of the cytoskeleton.

Synonyms

Enhancer of filamentation 1;hEF1;CRK-associated substrate-related protein;CAS-L;CasL;Cas scaffolding protein family member 2;Neural precursor cell expressed developmentally down-regulated protein 9;NEDD-9;Renal carcinoma antigen NY-REN-12;p105;Enhancer of filamentation 1 p55;NEDD9;CASL, CASS2;

Gene Name

Enhancer of filamentation 1

UniProt

Q14511

Host

Rabbit

Reactivity

Human,Monkey,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human HEF1 recombinant protein (Position: K273-E421). Human HEF1 shares 83% amino acid (aa) sequence identity with mouse HEF1.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Higher levels detected in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines. The protein has been detected in lymphocytes, in diverse cell lines, and in lung tissues.

Applications

Flow Cytometry,IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat<br> Immunohistochemistry, 2-5μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Subcellular Location

Cytoplasm, cell cortex. Nucleus. Golgi apparatus. Cell projection, lamellipodium. Cytoplasm. Cell junction, focal adhesion. Localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells localized predominantly in the cell periphery with particular concentration in lamellipodia but is also found in the nucleus. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with mitotic spindle.

Sequence Similarities

Belongs to the CAS family.

Protein Name

Enhancer of filamentation 1