Anti-Leptin Antibody Picoband® (APC Conjugate)

About this product

Boster Bio <a href="https://www.bosterbio.com/anti-leptin-picoband-trade-antibody-a00479-4-boster.html" style="color:#EA8D28">Anti-Leptin Antibody</a> Picoband&reg; catalog # PB9658. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9658-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.

Synonyms

Leptin;Obesity factor;Lep;Ob;

Gene Name

Leptin

UniProt

P41160

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse Leptin, different from the related human sequence by five amino acids, and from the related rat sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Detected in blood plasma (PubMed:8820107). Produced and secreted by astrocytes (at protein level) (PubMed:19065001). Abundantly expressed in liver, brain and testis with highest levels in liver. In the brain, found in cerebellum, cerebral cortex, hippocampus and brain stem. Located to neurons and neuroglia. .

Applications

ELISA,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act ly or inly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

ELISA, 0.1-0.5μg/ml, -<br>Western blot, 0.1-0.5μg/ml, Mouse<br>

Subcellular Location

Secreted .

Sequence Similarities

Contains 1 HMG box DNA-binding domain.

Protein Name

Leptin