Anti-TLR1 Antibody Picoband® (APC Conjugate)

About this product

Boster Bio Anti-TLR1 Antibody Picoband® catalog # A00429-2. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00429-2-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Toll-like receptor 1 (TLR1), also called TIL or CD281 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. This gene is mapped to 4p14 by fluorescence in situ hybridization. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene.

Synonyms

Toll-like receptor 1; Toll/interleukin-1 receptor-like protein; TIL; CD281; TLR1; KIAA0012

Gene Name

toll-like receptor 1

UniProt

Q15399

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human TLR1 recombinant protein (Position: F350-D404).

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.

Applications

ELISA,Flow Cytometry,IHC,IHC-F,ICC,WB

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:21078852). Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml<br> Immunohistochemistry (Frozen Section), 0.5-1μg/ml<br> Immunocytochemistry, 0.5-1μg/ml<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells<br> ELISA, 0.1-0.5μg/ml<br>

Subcellular Location

Cell membrane.