Anti-PPCS Antibody Picoband® (monoclonal, 7G13), APC Conjugate

About this product

Boster Bio Anti-PPCS Antibody Picoband® (monoclonal, 7G13) catalog # M05567. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

M05567-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Phosphopantothenoylcysteine synthetase in humans is encoded by the PPCS gene. Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. By genomic sequence analysis, this PPCS gene ia mapped to chromosome 1.

Synonyms

Phosphopantothenate--cysteine ligase; Phosphopantothenoylcysteine synthetase; PPC synthetase

Gene Name

phosphopantothenoylcysteine synthetase

Gene ID

79717

UniProt

Q9HAB8

Host

Mouse

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human PPCS, which shares 73.9% and 78.2% amino acid (aa) sequence identity with mouse and rat PPCS, respectively.

Clonality

Monoclonal

Clone

Clone: 7G13

Applications

Flow Cytometry,IHC,WB

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Function

Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

References & Citations

1. Daugherty, M., Polanuyer, B., Farrell, M., Scholle, M., Lykidis, A., de Crecy-Lagard, V., Osterman, A. Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics. J. Biol. Chem. 277: 21431-21439, 2002. 2. McHugh T, Laforce R, Gallagher P, Quinn S, Diggle P, Buchanan L (2006). "Natural history of the long-term cognitive, affective, and physical sequelae of a minor traumatic brain injury". Brain and Cognition. 60 (2): 209–11. 3. Bigler ED (2008). "Neuropsychology and clinical neuroscience of persistent post-concussive syndrome". Journal of the International Neuropsychological Society.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

34 kDa

Fragment

Mouse IgG2a

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat<br> Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human<br>

Subcellular Location

Cytosol.