Anti-PHF21B Antibody Picoband®, APC Conjugate

About this product

Boster Bio Anti-PHF21B Antibody Picoband® catalog # A16476-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A16476-1-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PHF21B (PHD finger protein 21B), also known as PHF4, is a 531 amino acid protein that contains one PHD-type zinc finger and is expressed as multiple alternatively spliced isoforms. The gene encoding PHF21B maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, neurofibromatosis type 2, autism and schizophrenia.

Synonyms

Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489

Gene Name

kelch repeat and BTB domain containing 2

Gene ID

112885

UniProt

Q96EK2

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PHF21B recombinant protein (Position: E291-M499).

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

WB,ICC,IF,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

References & Citations

1. Bergamo, N. A., da Silva Veiga, L. C., dos Reis, P. P., Nishimoto, I. N., Magrin, J., Kowalski, L. P., Squire, J. A., Rogatto, S. R. Classic and molecular cytogenetic analyses reveal chromosomal gains and losses correlated with survival in head and neck cancer patients. Clin. Cancer Res. 11: 621-631, 2005. 2. Bertonha, F. B., Barros Filho, M. deC., Kuasne, H., dos Reis, P. P., da Costa Prando, E., Munoz, J. J. A. M., Roffe, M., Hajj, G. N. M., Kowalski, L. P., Rainho, C. A., Rogatto, S. R. PHF21B as a candidate tumor suppressor gene in head and neck squamous cell carcinomas. Molec. Oncol. 9: 450-462, 2015. 3. Reis, P. P., Rogatto, S. R., Kowalski, L. P., Nishimoto, I. N., Montovani, J. C., Corpus, G., Squire, J. A., Kamel-Reid, S. Quantitative real-time PCR identifies a critical region of deletion on 22q13 related to prognosis in oral cancer. Oncogene 21: 6480-6487, 2002.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

24145 MW

Observed Molecular Weight

57 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br> ELISA, 0.1-0.5μg/ml, -<br>

Subcellular Location

Cul3-RING ubiquitin ligase complex.

Protein Name

Transmembrane protein 240