Anti-PSMD8 Antibody Picoband®, APC Conjugate

About this product

Boster Bio Anti-PSMD8 Antibody Picoband® catalog # A12465-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A12465-1-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

26S proteasome non-ATPase regulatory subunit 8 is an enzyme that in humans is encoded by the PSMD8 gene. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1.

Synonyms

RNA-binding motif, single-stranded-interacting protein 3; RBMS3

Gene Name

RNA binding motif single stranded interacting protein 3

Gene ID

5714

UniProt

P48556

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PSMD8 recombinant protein (Position: A86-V350).

Clonality

Polyclonal

Tissue Specificity

Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Applications

WB,IHC,ICC,IF,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Binds poly (A) and poly (U) oligoribonucleotides.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Hartz, P. A. Personal Communication. Baltimore, Md. 1/23/2018. 2. Kominami, K., DeMartino, G. N., Moomaw, C. R., Slaughter, C. A., Shimbara, N., Fujimoro, M., Yokosawa, H., Hisamatsu, H., Tanahashi, N., Shimizu, Y., Tanaka, K., Toh-e, A. Nin1p, a regulatory subunit of the 26S proteosome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae. EMBO J. 14: 3105-3115, 1995.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

47 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human<br> Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Cytoplasm.