Anti-PPP1R36 Antibody Picoband®, APC Conjugate

About this product

Boster Bio Anti-PPP1R36 Antibody Picoband® catalog # A17066-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A17066-1-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

PPP1R36 (protein phosphatase 1, regulatory subunit 36) is a protein-coding gene. GO annotations related to this gene include phosphatase binding and protein phosphatase inhibitor activity.

Synonyms

Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489

Gene Name

kelch repeat and BTB domain containing 2

Gene ID

145376

UniProt

Q96LQ0

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PPP1R36 recombinant protein (Position: R44-D381).

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Gross, M. B. Personal Communication. Baltimore, Md. 6/16/2022. 2. Gross, N., Strillacci, M. G., Penagaricano, F., Khatib, H. Characterization and functional roles of paternal RNAs in 2-4 cell bovine embryos. Sci. Rep. 9: 20347, 2019. 3. Zhang, Q., Gao, M., Zhang, Y., Song, Y., Cheng, H., Zhou, R. The germline-enriched Ppp1r36 promotes autophagy. Sci. Rep. 6: 24609, 2016.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

24145 MW

Observed Molecular Weight

54 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Cul3-RING ubiquitin ligase complex.

Protein Name

Transmembrane protein 240