Anti-PRSS35 Antibody Picoband®, APC Conjugate

About this product

Boster Bio Anti-PRSS35 Antibody Picoband® catalog # A14920-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A14920-1-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Proteolytic degradation of extracellular matrix components has been suggested to play an essential role for the occurrence of ovulation. The plasminogen activator and matrix metalloproteinase systems, which were previously believed to be crucial for ovulation, are not required in this process. PRSS35, which was upregulated by gonadotropins. PRSS23 was highly expressed in atretic follicles and it was expressed in the ovarian stroma and theca tissues just prior to ovulation. PRSS35 was expressed in the theca layers of developing follicles. It was also highly induced in granulosa cells of preovulatory follicles. PRSS35 was also expressed in the forming and regressing CL. PRSS35 may be involved in ovulation and CL formation and regression, and that PRSS23 may play a role in follicular atresia.

Synonyms

Transmembrane protein 240;TMEM240;C1orf70;

Gene Name

Transmembrane protein 240

Gene ID

167681

UniProt

Q8N3Z0

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human PRSS35 recombinant protein (Position: H30-D340).

Clonality

Polyclonal

Tissue Specificity

Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Applications

WB,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Miyakoshi, K. , Murphy, M. J. , Yeoman, R. R. , Mitra, S. , Dubay, C. J. , & Hennebold, J. D. . (2006). The identification of novel ovarian proteases through the use of genomic and bioinformatic methodologies. Biology of Reproduction, 75(6), 823-35. 2. Floyd, Brendan, J. , Wilkerson, Emily, M. , Veling, & Mike, T. , et al. Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function. 3. Clark, H. F. , Gurney, A. L. , Abaya, E. , Baker, K. , Baldwin, D. , & Brush, J. , et al. (2003). The secreted protein discovery initiative (spdi), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome Research, 13(10), 2265-2276.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

19908 MW

Observed Molecular Weight

53 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Rat<br> ELISA, 0.1-0.5 μg/ml, -<br>

Subcellular Location

Cell junction, synapse . Cell membrane ; Multi-pass membrane protein .

Protein Name

Transmembrane protein 240