Anti-Interleukin-10 IL10 Antibody Picoband®, APC Conjugate

About this product

Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1015. Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1015-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Synonyms

Interleukin-10;IL-10;Cytokine synthesis inhibitory factor;CSIF;Il10;Il-10;

Gene Name

Interleukin-10

UniProt

P18893

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived mouse IL-10 recombinant protein (Position: S19-S178).

Clonality

Polyclonal

Tissue Specificity

Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Applications

ELISA,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Components

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

References & Citations

1. Eskdale J, Kube D, Tesch H, Gallagher G (1997). "Mapping of the human IL10 gene and further characterization of the 5' flanking sequence".

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

20641 MW

Observed Molecular Weight

19 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

ELISA, 0.1-0.5μg/ml, -<br>Western blot, 0.1-0.5μg/ml, Mouse<br>

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the IL-10 family.

Protein Name

Interleukin-10(IL-10)