Anti-5HT1A Receptor/HTR1A Antibody Picoband®, APC Conjugate

About this product

Boster Bio Anti-5HT1A Receptor/HTR1A Antibody catalog # PA1647. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1647-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

HTR1A (5-HYDROXYTRYPTAMINE RECEPTOR 1A), also called SEROTONIN 5-HT-1A RECEPTOR or BETA-2-ADRENERGIC RECEPTOR-LIKE PROTEIN G-21, is a subtype of 5-HT receptor that binds the endogenous neurotransmitter serotonin. It is a G protein-coupled receptor (GPCR) that is coupled to Gi/Go and mediates inhibitory neurotransmission. HTR1A denotes the human gene encoding for the receptor.The HTR1A gene is located at 5q12.3. The decreases in 5-HT-1A receptor densities correlated with decreased glucose utilization as measured by PET scan. Activation of 5-HT-1A receptors has been demonstrated to impair cognition, learning, and memory by inhibiting the release of glutamate and acetylcholine in various areas of the brain. 5-HT-1A receptors in the dorsal raphe nucleus are co-localized with neurokinin 1 (NK1) receptors and have been shown to inhibit the release of substance P, their endogenous ligand.

Synonyms

5-hydroxytryptamine receptor 1A;5-HT-1A;5-HT1A;G-21;Serotonin receptor 1A;HTR1A;ADRB2RL1, ADRBRL1;

Gene Name

5-hydroxytryptamine receptor 1A

UniProt

P08908

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT1A Receptor, different from the related rat and mouse sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Detected in lymph nodes, thymus and spleen. Detected in activated T-cells, but not in resting T-cells. .

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca (2+) ions from intracellular stores. Plays a role in the regulation of 5- hydroxytryptamine release and in the regulation of dopamine and 5- hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

References & Citations

1. Gilliam TC, Freimer NB, Kaufmann CA, Powchik PP, Bassett AS, Bengtsson U, Wasmuth JJ (November 1989). "Deletion Mapping of DNA Markers to a Region of Chromosome 5 That Cosegregates with Schizophrenia". Genomics 5 (4): 940–4. 2. Audero, E., Coppi, E., Mlinar, B., Rossetti, T., Caprioli, A., Banchaabouchi, M. A., Corradetti, R., Gross, C. Sporadic autonomic dysregulation and death associated with excessive serotonin autoinhibition. 3. El Mestikawy, S., Fargin, A., Raymond, J. R., Gozlan, H., Hnatowich, M. The 5-HT(1A) receptor: an overview of recent advances. Neurochem. Res. 16: 1-10, 1991.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

46107 MW

Observed Molecular Weight

46 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat, By Heat<br>

Subcellular Location

Cell membrane ; Multi-pass membrane protein .

Sequence Similarities

Belongs to the WD repeat BUB3 family.

Protein Name

5-hydroxytryptamine receptor 1A(5-HT-1A/5-HT1A)