- Catalog number
- PA1683-APC
- Size / count
- 100 µg/Vial
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- SLC22A6 (Solute carrier family 22 (organic anion transporter), member 6), also called OAT1 or PAHT, is a protein that in humans is encoded by the SLC22A6 gene, which is also a member of the organic anion transporter (OAT) family of proteins. OAT1 is a transmembrane protein that is expressed in the brain, the placenta, the eyes, smooth muscles, and the basolateral membrane of proximal tubular cells of the kidneys. The SLC22A6 gene is mapped on 11q12.3. It plays a central role in renal organic anion transport. Along with OAT3, OAT1 mediates the uptake of a wide range of relatively small and hydrophilic organic anions from plasma into the cytoplasm of the proximal tubular cells of the kidneys. The SLC22A6 gene contains 10 exons and spans 8.2 kb. OAT1 functions as organic anion exchanger. When the uptake of one molecule of an organic anion is transported into a cell by an OAT1 exchanger, one molecule of an endogenous dicarboxylic acid (such as glutarate, ketoglutarate, etc) is simultaneously transported out of the cell. PAH uptake in Xenopus oocytes injected with OAT1 mRNA was demonstrated by Race et al.
- Synonyms
- Solute carrier family 22 member 6;Organic anion transporter 1;hOAT1;PAH transporter;hPAHT;Renal organic anion transporter 1;hROAT1;SLC22A6;OAT1, PAHT;
- Gene Name
- Solute carrier family 22 member 6
- UniProt
- Q4U2R8
- Host
- Rabbit
- Reactivity
- Human,Rat
- Cross Reactivity
- No cross-reactivity with other proteins
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human SLC22A6, different from the related rat sequence by three amino acids.
- Clonality
- Polyclonal
- Tissue Specificity
- Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule. .
- Applications
- IHC,WB
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Function
- Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) (By similarity). Mediates the sodium-independent uptake of p- aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido- 3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro- phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2- furanpropionate (CMPF), cidofovir, adefovir, 9- (2- phosphonylmethoxyethyl) guanine (PMEG), 9- (2- phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p- chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid (By similarity). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate. .
- Components
- Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
- References & Citations
- 1. Bahn, A., Prawitt, D., Buttler, D., Reid, G., Enklaar, T., Wolff, N. A., Ebbinghaus, C., Hillemann, A., Schulten, H.-J., Gunawan, B., Fuzesi, L., Zabel, B., Burckhardt, G. Genomic structure and in vivo expression of the human organic anion transporter 1 (hOAT1) gene. Biochem. Biophys. Res. Commun. 275: 623-630, 2000.
2. Race, J. E., Grassl, S. M., Williams, W. J., Holtzman, E. J. Molecular cloning and characterization of two novel human renal organic anion transporters (hOAT1 and hOAT3). Biochem. Biophys. Res. Commun. 255: 508-514, 1999.
- Storage Conditions
- Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
- Calculated Molecular Weight
- 61816 MW
- Observed Molecular Weight
- 36 kDa
- Fragment
- Rabbit IgG
- Specificity
- No cross reactivity with other proteins.
- Applications Notes
- Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat<br>Western blot, 0.1-0.5μg/ml, Human<br>
- Subcellular Location
- Cell membrane ; Multi-pass membrane protein .
- Sequence Similarities
- Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.
- Protein Name
- Solute carrier family 22 member 6
