Anti-Vinculin/VCL Antibody Picoband®, APC Conjugate

About this product

Boster Bio Anti-Vinculin/VCL Antibody catalog # PA1781. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1781-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Synonyms

Vinculin;Metavinculin;MV;VCL;

Gene Name

Vinculin

UniProt

P18206

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Metavinculin is muscle-specific.

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Ezzell RM, Goldmann WH, Wang N, Parashurama N, Parasharama N, Ingber DE (1997). "Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton". Exp. Cell Res. 231 (1): 14–26. 2. Goldmann WH, Ingber DE (2001). "Intact vinculin protein is Require for control of cell shape, cell mechanics, and rac-Dependent Lamellipodia Formation". Biochemical and Biophysical Research Communications. 290 (2): 749–755. 3. Xu W, Baribault H, Adamson ED (1998). "vinculin knockout results in heart and brain defects during embryonic development". Development. 125(2): 327–337.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

123799 MW

Observed Molecular Weight

34 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat<br>Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br>

Subcellular Location

Cytoplasm, cytoskeleton . Cell junction, adherens junction . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, focal adhesion . Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity). Colocalizes with LIMD1 in the focal adhesions. .

Sequence Similarities

Belongs to the vinculin/alpha-catenin family.

Protein Name

Vinculin