Anti-LIM and SH3 domain protein 1 LASP1 Antibody Picoband®, APC Conjugate

About this product

Boster Bio Anti-LIM and SH3 domain protein 1 LASP1 Antibody catalog # PA1791-1. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1791-1-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

LASP1 (LIM and SH3 domain protein 1), also known as LASP1, is a protein that in humans is encoded by the LASP1 gene. This gene encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization. LASP1 has been shown to interact with Zyxin. Tomasetto et al. (1995) mapped the LASP1 gene to chromosome 17q11-q21.3 by radioactive in situ hybridization. Northern blot analysis revealed that LASP1 mRNA was expressed at a basal level in all normal tissues examined and overexpressed in 8% of primary breast cancers. In most of these cancers, LASP1 and ERBB2 were simultaneously overexpressed.

Synonyms

LIM and SH3 domain protein 1;LASP-1;Metastatic lymph node gene 50 protein;MLN 50;LASP1;MLN50;

Gene Name

LIM and SH3 domain protein 1

UniProt

Q14847

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human LASP1.

Clonality

Polyclonal

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity). .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Schreiber, V., Masson, R., Linares, J. L., Mattei, M. G., Tomasetto, C., Rio, M. C.Chromosomal assignment and expression pattern of the murine Lasp-1 gene.Gene 207: 171-175, 1998. 2. Tomasetto, C., Moog-Lutz, C., Regnier, C. H., Schreiber, V., Basset, P., Rio, M.-C.Lasp-1 (MLN 50) defines a new LIM protein subfamily characterized by the association of LIM and SH3 domains.FEBS Lett. 373: 245-249, 1995. 3. Tomasetto, C., Regnier, C., Moog-Lutz, C., Mattei, M. G., Chenard, M. P., Lidereau, R., Basset, P., Rio, M. C.Identification of four novel human genes amplified and overexpressed in breast carcinoma and localized to the q11-q21.3 region of chromosome 17.Genomics 28: 367-376, 1995.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

29717 MW

Observed Molecular Weight

11 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat<br>Western blot, 0.1-0.5μg/ml, Human<br>

Subcellular Location

Cytoplasm, cell cortex . Cytoplasm, cytoskeleton . Associated with the F- actin rich cortical cytoskeleton. .

Sequence Similarities

Contains 1 LIM zinc-binding domain.

Protein Name

LIM and SH3 domain protein 1