Anti-GCN2/EIF2AK4 Antibody Picoband®, APC Conjugate

About this product

Boster Bio Anti-GCN2/EIF2AK4 Antibody catalog # PA2028. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2028-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

EIF2AK4 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4), also called GCN2, is an enzyme that in humans is encoded by the EIF2AK4 gene. EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses. Hartz (2005) mapped the EIF2AK4 gene to chromosome 15q15.1 based on an alignment of the EIF2AK4 sequence with the genomic sequence. Berlanga et al. (1999) demonstrated that Gcn2 immunopurified from mouse liver extracts could phosphorylate rabbit Eif2 in vitro. Serum starvation increased the level of phosphorylated EIF2-alpha more than 2-fold in human embryonic kidney cells transfected with mouse Eif2ak4. Costa-Mattioli et al. (2005) reported a unique feature of hippocampal slices from Gcn2-null mice: in CA1, a single 100-Hz train induced a strong and sustained long-term potentiation (late LTP or L-LTP), which was dependent on transcription and translation.

Synonyms

Eukaryotic translation initiation factor 2-alpha kinase 4;2.7.11.1;GCN2-like protein;mGCN2;Eif2ak4;Gcn2, Kiaa1338;

Gene Name

Eukaryotic translation initiation factor 2-alpha kinase 4

UniProt

Q9QZ05

Host

Rabbit

Reactivity

Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse GCN2, different from the related rat sequence by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Expressed in liver (at protein level). Widely expressed, with the highest levels in the liver and the brain. Isoform 4 is expressed in brain followed by testis and liver and isoform 3 is expressed only in brain. .

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Can phosphorylate the alpha subunit of EIF2 and may mediate translational control.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Berlanga, J. J., Santoyo, J., de Haro, C. Characterization of a mammalian homolog of the GCN2 eukaryotic initiation factor 2-alpha kinase. Europ. J. Biochem. 265: 754-762, 1999. 2. Costa-Mattioli, M., Gobert, D., Harding, H., Herdy, B., Azzi, M., Bruno, M., Bidinosti, M., Ben Mamou, C., Marcinkiewicz, E., Yoshida, M., Imataka, H., Cuello, A. C., Seidah, N., Sossin, W., Lacaille, J.-C., Ron, D., Nader, K., Sonenberg, N. Translational control of hippocampal synaptic plasticity and memory by the elF2-alpha kinase GCN2. Nature 436: 1166-1170, 2005. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 3/28/2005.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

186487 MW

Observed Molecular Weight

94 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Mouse, Rat<br>

Sequence Similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.

Protein Name

eIF-2-alpha kinase GCN2