Anti-RGS9 Antibody Picoband®, APC Conjugate

About this product

Boster Bio Anti-RGS9 Antibody catalog # PA2241. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2241-APC

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

Regulator of G-protein signaling 9, also known as RGS9 is a human gene, which codes for a protein involved in regulation of signal transduction inside cells. This gene is mapped to 17q24.1. This gene encodes a member of the RGS family of GTPase activating proteins that function in various signaling pathways by accelerating the deactivation of G proteins. This protein is anchored to photoreceptor membranes in retinal cells and deactivates G proteins in the rod and cone phototransduction cascades. Mutations in this gene result in bradyopsia. Multiple transcript variants encoding different isoforms have been found for this gene.

Synonyms

Regulator of G-protein signaling 9;RGS9;RGS9;

Gene Name

Regulator of G-protein signaling 9

UniProt

O75916

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human RGS9, different from the related mouse and rat sequences by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in the caudate and putamen, lower levels found in the hypothalamus and nucleus accumbens and very low levels in cerebellum. Not expressed in globus pallidus or cingulate cortex. Isoform 2 is expressed predominantly in pineal gland and retina. Isoform 3 is expressed in retina (abundant in photoreceptors).

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G (t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction (By similarity). .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Cabrera-Vera, T. M., Hernandez, S., Earls, L. R., Medkova, M., Sundgren-Andersson, A. K., Surmeier, D. J., Hamm, H. E. RGS9-2 modulates D2 dopamine receptor-mediated Ca(2+) channel inhibition in rat striatal cholinergic interneurons. Proc. Nat. Acad. Sci. 101: 16339-16344, 2004. 2. Hu, G., Zhang, Z., Wensel, T. G. Activation of RGS9-1GTPase acceleration by its membrane anchor, R9AP. J. Biol. Chem. 278: 14550-14554, 2003. 3. Sierra, D. A., Gilbert, D. J., Householder, D., Grishin, N. V., Yu, K., Ukidwe, P., Barker, S. A., He, W., Wensel, T. G., Otero, G., Brown, G., Copeland, N. G., Jenkins, N. A., Wilkie, T. M. Evolution of the regulators of G-protein signaling multigene family in mouse and human. Genomics 79: 177-185, 2002.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

76966 MW

Observed Molecular Weight

56 kDa, 77 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br>

Subcellular Location

Isoform 3: Membrane; Peripheral membrane protein. Isoform 3 is targeted to the membrane via its interaction with RGS9BP. .

Sequence Similarities

Contains 1 DEP domain.

Protein Name

Regulator of G-protein signaling 9