Anti-TRPM5 Antibody Picoband® (iFluor647 Conjugate)

About this product

Boster Bio Anti-TRPM5 Antibody Picoband® catalog # PB9903. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9903-iFluor647

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Transient receptor potential cation channel subfamily M member 5 (TRPM5) is a protein that in humans is encoded by the TRPM5 gene. This gene encodes a member of the transient receptor potential (TRP) protein family, which is a diverse group of proteins with structural features typical of ion channels. This protein plays an important role in taste transduction, and has characteristics of a calcium-activated, non-selective cation channel that carries Na+, K+, and Cs+ ions equally well, but not Ca (2+) ions. It is activated by lower concentrations of intracellular Ca (2+), and inhibited by higher concentrations. It is also a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. This gene is located within the Beckwith-Wiedemann syndrome critical region-1 on chromosome 11p15.5, and has been shown to be imprinted, with exclusive expression from the paternal allele.

Synonyms

Transient receptor potential cation channel subfamily M member 5;Long transient receptor potential channel 5;LTrpC-5;LTrpC5;MLSN1- and TRP-related gene 1 protein;TRPM5 ;LTRPC5 , MTR1 ;

Gene Name

Transient receptor potential cation channel subfamily M member 5

UniProt

Q9NZQ8

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human TRPM5 recombinant protein (Position: S1021-Q1108). Human TRPM5 shares 87.5% amino acid (aa) sequence identity with mouse TRPM5.

Clonality

Polyclonal

Tissue Specificity

Strongly expressed in fetal brain, liver and kidney, and in adult prostate, testis, ovary, colon and peripheral blood leukocytes. Also expressed in a large proportion of Wilms' tumors and rhabdomyosarcomas. In monochromosomal cell lines shows exclusive paternal expression. .

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Voltage-modulated Ca (2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent- specific, non-selective cation channel that mediates the transport of Na (+), K (+) and Cs (+) ions equally well. Activated ly by increases in intracellular Ca (2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca (2+). Also activated by a fast intracellular Ca (2+) increase in response to inositol 1,4,5- triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage- dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat <br>Western blot, 0.1-0.5μg/ml, Human<br>

Subcellular Location

Cell membrane ; Multi-pass membrane protein .

Protein Name

Transient receptor potential cation channel subfamily M member 5