Anti-CIC Antibody -iFluor647 Conjugate

About this product

Boster Bio Anti-CIC Antibody Picoband® catalog # A00385-1. Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A00385-1-iFluor647

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Protein capicua homolog is a protein that in humans is encoded by the CIC gene. The protein encoded by this gene is an ortholog of the Drosophila melanogaster capicua gene, and is a member of the high mobility group (HMG)-box superfamily of transcriptional repressors. This protein contains a conserved HMG domain that is involved in DNA binding and nuclear localization, and a conserved C-terminus. Studies suggest that the N-terminal region of this protein interacts with Atxn1 (GeneID:6310), to form a transcription repressor complex, and in vitro studies suggest that polyglutamine-expansion of ATXN1 may alter the repressor activity of this complex. Mutations in this gene have been associated with olidogdendrogliomas (PMID:21817013). In addition, translocation events resulting in gene fusions of this gene with both DUX4 (GeneID:100288687) and FOXO4 (GeneID:4303) have been associated with round cell sarcomas. This gene is mapped to 19q13.2. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms

Protein capicua homolog; CIC; KIAA0306

Gene Name

capicua transcriptional repressor

Gene ID

23152

UniProt

Q96RK0

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E. coli-derived human CIC recombinant protein (Position: E1433-Q1527).

Clonality

Polyclonal

Tissue Specificity

Expressed in fetal brain.

Applications

ELISA,IHC

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcriptional repressor which plays a role in development of the central nervous system (CNS). In concert with ATXN1 and ATXN1L, involved in brain development.

Components

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

References & Citations

1. Lee, C.-J., Chan, W.-I., Scotting, P. J. CIC, a gene involved in cerebellar development and ErbB signaling, is significantly expressed in medulloblastomas. J. Neurooncol. 73: 101-108, 2005. 2. Lu, H.-C., Tan, Q., Rousseaux, M. W. C., Wang, W., Kim, J.-Y., Richman, R., Wan, Y.-W., Yeh, S.-Y., Patel, J. M., Liu, X., Lin, T., Lee, Y., and 25 others.Disruption of the ATXN1-CIC complex causes a spectrum of neurobehavioral phenotypes in mice and humans. Nature Genet. 49: 527-536, 2017.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

55 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml<br> ELISA, 0.1-0.5μg/ml<br>

Subcellular Location

Nucleus.