Anti-TMEM65 Antibody Picoband®, iFluor647 Conjugate

About this product

Boster Bio Anti-TMEM65 Antibody Picoband® catalog # A13650-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A13650-1-iFluor647

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Predicted to be involved in cardiac ventricle development and regulation of cardiac conduction. Located in intercalated disc; mitochondrial inner membrane; and plasma membrane.

Synonyms

RNA-binding protein 47;RNA-binding motif protein 47;RBM47;

Gene Name

RNA-binding protein 47

Gene ID

157378

UniProt

Q6PI78

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human TMEM65, identical to the related mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

Applications

WB,Flow Cytometry

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Hartz, P. A. Personal Communication. Baltimore, Md. 10/23/2015. 2. Nishimura, N., Gotoh, T., Oike, Y., Yano, M. TMEM65 is a mitochondrial inner-membrane protein. PeerJ 2: e349, 2014. Note: Electronic Article. 3. Sharma, P., Abbasi, C., Lazic, S., Teng, A. C. T., Wang, D., Dubois, N., Ignatchenko, V., Wong, V., Liu, J., Araki, T., Tiburcy, M., Ackerly, C., and 9 others. Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function. Nature Commun. 6: 8391, 2015. Note: Electronic Article.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

64099 MW

Observed Molecular Weight

20 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat<br> Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat<br>

Subcellular Location

Nucleus .

Protein Name

Follicular dendritic cell secreted peptide