- Catalog number
- A10688-1-iFluor647
- Size / count
- 100 µg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- Yes
- Background
- PDE12 is an enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. It also cleaves 3',5'-phosphodiester bond of oligoadenylates and plays a role as a negative regulator of the The 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.
- Synonyms
- 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody
- Gene Name
- Serine/threonine-protein kinase AtPK1/AtPK6
- Gene ID
- 201626
- UniProt
- Q6L8Q7
- Host
- Rabbit
- Reactivity
- Human,Mouse,Rat
- Cross Reactivity
- No cross reactivity with other proteins.
- Immunogen
- E.coli-derived human PDE12 recombinant protein (Position: E36-K607). Human PDE12 shares 89.9% and 91.3% amino acid (aa) sequence identity with mouse and rat PDE12, respectively.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in all tissues.
- Applications
- WB,Flow Cytometry,ELISA
- Purification
- Immunogen affinity purified.
- Concentration
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Form
- Lyophilized
- Reconstitution
- Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- Function
- Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.
- Components
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
- References & Citations
- 1. Kubota, K., Nakahara, K., Ohtsuka, T., Yoshida, S., Kawaguchi, J., Fujita, Y., Ozeki, Y., Hara, A., Yoshimura, C., Furukawa, H., Haruyama, H., Ichikawa, K., Yamashita, M., Matsuoka, T., Iijima, Y. Identification of 2-prime-phosphodiesterase, which plays a role in the 2-5A system regulated by interferon. J. Biol. Chem. 279: 37832-37841, 2004.
2. Rorbach, J., Nicholls, T. J. J., Minczuk, M. PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human mitochondria. Nucleic Acids Res. 39: 7750-7763, 2011.
3. Wood, E. R., Bledsoe, R., Chai, J., Daka, P., Deng, H., Ding, Y., Harris-Gurley, S., Kryn, L. H., Nartey, E., Nichols, J., Nolte, R. T., Prabhu, N., and 12 others. The role of phosphodiesterase 12 (PDE12) as a negative regulator of the innate immune response and the discovery of antiviral inhibitors. J. Biol. Chem. 290: 19681-19696, 2015.
- Storage Conditions
- At -20℃ for one year from date of receipt.
After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months.
Avoid repeated freezing and thawing.
- Calculated Molecular Weight
- 52588 MW
- Observed Molecular Weight
- 70 kDa
- Fragment
- IgG
- Applications Notes
- Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat<br>
Flow Cytometry (Fixed), 1-3 μg/1x10<sup>6</sup> cells, Human<br>
ELISA, 0.1-0.5 μg/ml, -<br>
- Subcellular Location
- Cytoplasm. Nucleus.
- Protein Name
- Serine/threonine-protein kinase AtPK1/AtPK6
