Anti-NXPH3 Antibody Picoband®, iFluor647 Conjugate

About this product

Boster Bio Anti-NXPH3 Antibody Picoband® catalog # A14597-3. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A14597-3-iFluor647

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Neurexophilin-3 is a protein that in humans is encoded by the NXPH3 gene. Neurexophilin-3 (NXPH-3) is a neuropeptide-like secreted glycoprotein in the neurexophilin family. The 252 amino acid (aa) NXPH-3 precursor contains a 22 aa signal peptide, plus a 230 aa proprecursor that is likely cleaved at a basic motif, producing a 76 aa propeptide and a 154 aa mature protein. Mature rat NXPH-3 shares 99%, 95% and 92% aa identity with mouse, human and bovine NXPH-3, respectively. It contains motifs that are conserved among all neurexophilins, including three potential N-glycosylation sites in the N-terminal portion and six cysteines in the C-terminal portion. NXPH-3 is expressed selectively in subplate-derived neurons in the cortex, granule cells in the vestibulocerebellum, and Cajal-Retzius cells during development. NXPH-1 is the neurexophilin most similar to NXPH-3, sharing 69% aa identity within the mature region. Expression of NXPH-1 and NXPH-3 does not appear to overlap, with NXPH-1 expression occurring mainly by cells that resemble inhibitory interneurons. Both are tightly bound extracellular ligands of alpha -neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release. Genetic deletion of NXPH-1 and/or NXPH-3 produces no anatomical effect, although mice lacking NXPH-3 show specific defects in motor coordination. Of the other known neurexophilins, NXPH-2 is not expressed in rodents, and NXPH-4 does not bind alpha -neurexins.

Gene Name

NXPH3

Gene ID

11248

UniProt

O95157

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

E.coli-derived human NXPH3 recombinant protein (Position: R5-K217). Human NXPH3 shares 95.8% amino acid (aa) sequence identity with mouse and rat NXPH3, respectively.

Clonality

Polyclonal

Applications

WB,IHC,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Gross, M. B. Personal Communication. Baltimore, Md. 10/24/2014. 2. Missler, M., Sudhof, T. C. Neurexophilins form a conserved family of neuropeptide-like glycoproteins. J. Neurosci. 18: 3630-3638, 1998. 3. Petrenko, A. G., Ullrich, B., Missler, M., Krasnoperov, V., Rosahl, T. W., Sudhof, T. C. Structure and evolution of neurexophilin. J. Neurosci. 16: 4360-4369, 1996.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Observed Molecular Weight

28 kDa

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human<br> Immunohistochemistry, 2-5 μg/ml, Mouse, Rat<br> Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human<br> Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human<br> ELISA, 0.1-0.5 μg/ml, -<br>