Anti-Interleukin-10 IL10 Antibody Picoband®, iFluor647 Conjugate

About this product

Boster Bio Anti-Interleukin-10 IL10 Antibody catalog # RP1016. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

RP1016-iFluor647

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Interleukin-10 (IL-10 or IL10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans IL-10 is encoded by the IL10 gene. It is capable of inhibiting synthesis of pro-inflammatory cytokines like IFN-gamma, IL-2, IL-3, TNFalpha and GM-CSF made by cells such as macrophages and regulatory T-cells.IL-10 also displays potent abilities to suppress the antigen presentation capacity of antigen presenting cells. Kim et al. (1992) showed that the mouse IL 10 gene contains 5 exons and spans about 5.2 kb of genomic DNA. Eskdale et al. (1997) mapped the IL10 gene to the junction between 1q31 and 1q32.

Synonyms

Interleukin-10;IL-10;Cytokine synthesis inhibitory factor;CSIF;Il10;Il-10;

Gene Name

Interleukin-10

UniProt

P29456

Host

Rabbit

Reactivity

Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E. coli-derived rat IL-10 recombinant protein (Position: S19-N178).

Clonality

Polyclonal

Tissue Specificity

Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Applications

ELISA,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. .

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

References & Citations

1. Eskdale J, Kube D, Tesch H, Gallagher G (1997). "Mapping of the human IL10 gene and further characterization of the 5' flanking sequence".

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

20426 MW

Observed Molecular Weight

21 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat, By Heat <br>ELISA, 0.1-0.5μg/ml, -<br>

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the IL-10 family.

Protein Name

Interleukin-10(IL-10)