Anti-HSD17B1 Antibody Picoband®, iFluor647 Conjugate

About this product

Boster Bio Anti-HSD17B1 Antibody catalog # PA1613-1. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1613-1-iFluor647

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Estradiol 17-beta-dehydrogenase 1 is an enzyme that in humans is encoded by the HSD17B1 gene. This gene encodes a member of the 17beta-hydroxysteroid dehydrogenase family of short-chain dehydrogenases/reductases. It has a dual function in estrogen activation and androgen inactivation and plays a major role in establishing the estrogen E2 concentration gradient between serum and peripheral tissues. The encoded protein catalyzes the last step in estrogen activation, using NADPH to convert estrogens E1 and E2 and androgens like 4-androstenedione, to testosterone. It has an N-terminal short-chain dehydrogenase domain with a cofactor binding site, and a narrow, hydrophobic C-terminal domain with a steroid substrate binding site. This gene is expressed primarily in the placenta and ovarian granulosa cells, and to a lesser extent, in the endometrium, adipose tissue, and prostate. Polymorphisms in this gene have been linked to breast and prostate cancer. A pseudogene of this gene has been identified. Alternative splicing results in multiple transcript variants.

Synonyms

Estradiol 17-beta-dehydrogenase 1;1.1.1.62;17-beta-hydroxysteroid dehydrogenase type 1;17-beta-HSD 1;20 alpha-hydroxysteroid dehydrogenase;20-alpha-HSD;E2DH;Placental 17-beta-hydroxysteroid dehydrogenase;Short chain dehydrogenase/reductase family 28C member 1;HSD17B1;E17KSR, EDH17B1, EDH17B2, EDHB17, SDR28C1;

Gene Name

Estradiol 17-beta-dehydrogenase 1

UniProt

P14061

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human HSD17B1, different from the related rat and mouse sequences by one amino acid.

Clonality

Polyclonal

Tissue Specificity

Detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. .

Applications

IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. "Entrez Gene: HSD17B1 Hydroxysteroid (17-beta) dehydrogenase 1". 2. Aka JA, Mazumdar M, Chen CQ, Poirier D, Lin SX (Apr 2010). "17beta-hydroxysteroid dehydrogenase type 1 stimulates breast cancer by dihydrotestosterone inactivation in addition to estradiol production".Molecular Endocrinology. 24 (4): 832–45.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34950 MW

Observed Molecular Weight

37-38 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human, Rat<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat<br> Immunocytochemistry , 0.5-1μg/ml, Human<br>

Subcellular Location

Cytoplasm.

Sequence Similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein Name

Estradiol 17-beta-dehydrogenase 1