Anti-Krueppel-like factor 5 KLF5 Antibody Picoband®, iFluor647 Conjugate

About this product

Boster Bio Anti-Krueppel-like factor 5 KLF5 Antibody catalog # PA1889. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA1889-iFluor647

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

KLF5 (Krueppel-like factor 5) is a protein that in humans is encoded by the KLF5 gene. It is also known as BASIC TRANSCRIPTION ELEMENT-BINDING PROTEIN 2 or BTEB2. This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Suske et al. (2005)stated that the human KLF5 gene maps to chromosome 13q21.33, and the mouse Klf5 gene to chromosome 14E2.1. Sogawa et al. (1993)isolated a cDNA encoding BTEB2. The predicted 219-amino acid protein contains 3 consecutive zinc finger motifs near the C terminus. The zinc finger domains were 59% and 64% identical to those of Sp1 (189906) and BTEB1, respectively. Both BTEB2 and Sp1 have a short basic region preceding the zinc finger motifs that may play an auxiliary role in DNA binding. The N-terminal region of BTEB2 is proline rich. Recombinant BTEB2 protein and Sp1 showed very similar DNA-binding specificity in vitro. When expressed in mammalian cells, BTEB2 activated the transcription of a <a href="https://www.bosterbio.com/cells/reporter-cell-lines" style="color:#ea8d28">reporter gene</a> fused to a GC box-containing promoter. Sogawa et al. (1993) also identified the rat BTEB2 homolog. Northern blot analysis of rat tissues revealed that BTEB2 is expressed only in the testis and placenta.

Synonyms

Krueppel-like factor 5;Basic transcription element-binding protein 2;BTE-binding protein 2;Colon krueppel-like factor;GC-box-binding protein 2;Intestinal-enriched krueppel-like factor;Transcription factor BTEB2;KLF5;BTEB2, CKLF, IKLF;

Gene Name

Krueppel-like factor 5

UniProt

Q13887

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human KLF5, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

Expressed only in testis and placenta.

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Oishi, Y., Manabe, I., Tobe, K., Tsushima, K., Shindo, T., Fujiu, K., Nishimura, G., Maemura, K., Yamauchi, T., Kubota, N., Suzuki, R., Kitamura, T., Akira, S., Kadowaki, T., Nagai, R.Kruppel-like transcription factor KLF5 is a key regulator of adipocyte differentiation.Cell Metab. 1: 27-39, 2005. 2. Shindo, T., Manabe, I., Fukushima, Y., Tobe, K., Aizawa, K., Miyamoto, S., Kawai-Kowase, K., Moriyama, N., Imai, Y., Kawakami, H., Nishimatsu, H., Ishikawa, T., and 10 others.Kruppel-like zinc-finger transcription factor KLF5/BTEB2 is a target for angiotensin II signaling and an essential regulator of cardiovascular remodeling.Nature Med. 8: 856-863, 2002. 3. Sogawa, K., Imataka, H., Yamasaki, Y., Kusume, H., Abe, H., Fujii-Kuriyama, Y.cDNA cloning and transcriptional properties of a novel GC box-binding protein, BTEB2.Nucleic Acids Res. 21: 1527-1532, 1993.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

50792 MW

Observed Molecular Weight

60 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human<br>

Subcellular Location

Nucleus .

Sequence Similarities

Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein Name

Krueppel-like factor 5