Anti-Prolactin Receptor/PRLR Antibody Picoband®, iFluor647 Conjugate

About this product

Boster Bio Anti-Prolactin Receptor/PRLR Antibody catalog # PA2087. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2087-iFluor647

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

PRLR (Prolactin Receptor), is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et al. (1989, 1990) demonstrated that the prolactin receptor gene resides in the same chromosomal region as the growth hormone receptor gene, which has been mapped to 5p13-p12. Cunningham et al. (1990) demonstrated that zinc greatly increases the affinity of GH for the extracellular binding domain of PRLR, although it is not required for binding of GH to the growth hormone receptor or for binding of prolactin to the prolactin receptor. By mutational analysis, they showed that a cluster of 3 residues (histidine-18, histidine-21, and glutamic acid-174) in GH and histidine-188 in PRLR (conserved in all PRL receptors but not GH receptors) are likely zinc-ion ligands.

Synonyms

Prolactin receptor;PRL-R;Prlr;

Gene Name

Prolactin receptor

UniProt

Q08501

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse PRLR, identical to the related rat sequence, and different from the related human sequence by three amino acids.

Clonality

Polyclonal

Tissue Specificity

Expressed abundantly in the testis. .

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

This is a receptor for the anterior pituitary hormone prolactin.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Arden, K. C., Boutin, J.-M., Djiane, J., Kelly, P. A., Cavenee, W. K. The receptors for prolactin and growth hormone are localized in the same region of human chromosome 5. Cytogenet. Cell Genet. 53: 161-165, 1990. 2. Arden, K. C., Cavenee, W. K., Boutin, J.-M., Kelly, P. A. The genes encoding the receptors for prolactin and growth hormone map to human chromosome 5. (Abstract) Am. J. Hum. Genet. 45 (suppl.): A129 only, 1989. 3. Cunningham, B. C., Bass, S., Fuh, G., Wells, J. A. Zinc mediation of the binding of human growth hormone to the human prolactin receptor. Science 250: 1709-1712, 1990.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

68241 MW

Observed Molecular Weight

95 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat<br>Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat<br>

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the type I cytokine receptor family. Type 1 subfamily.

Protein Name

Prolactin receptor