Anti-Solute carrier family 22 member 5/SLC22A5 Antibody Picoband®, iFluor647 Conjugate

About this product

Boster Bio Anti-Solute carrier family 22 member 5/SLC22A5 Antibody catalog # PA2174. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PA2174-iFluor647

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Solute carrier family 22 (organic cation/carnitine transporter), member 5, also called SLC22A5 or OCTN2 is a membrane transport protein associated with primary carnitine deficiency. This gene is mapped to 5q31.1. Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is a plasma integral membrane protein which functions both as an organic cation transporter and as a sodium-dependent high affinity carnitine transporter. The encoded protein is involved in the active cellular uptake of carnitine. Mutations in this gene are the cause of systemic primary carnitine deficiency (CDSP), an autosomal recessive disorder manifested early in life by hypoketotic hypoglycemia and acute metabolic decompensation, and later in life by skeletal myopathy or cardiomyopathy.

Synonyms

Solute carrier family 22 member 5;High-affinity sodium-dependent carnitine cotransporter;Organic cation/carnitine transporter 2;Slc22a5;Octn2;

Gene Name

Solute carrier family 22 member 5

UniProt

Q9Z0E8

Host

Rabbit

Reactivity

Rat,Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Solute carrier family 22 member 5, different from the related rat sequence by two amino acids.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Expressed in kidney, liver and testis. .

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Sodium-ion dependent, high affinity carnitine transporter. Involved in the active cellular uptake of carnitine. Transports one sodium ion with one molecule of carnitine. Also transports organic cations such as tetraethylammonium (TEA) without the involvement of sodium. Also relative uptake activity ratio of carnitine to TEA is 11.3.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Dobrowolski, S. F., McKinney, J. T., di San Filippo, C. A., Sim, K. G., Wilcken, B., Longo, N. Validation of dye-binding/high-resolution thermal denaturation for the identification of mutations in the SLC22A5 gene. Hum. Mutat. 25: 306-313, 2005. 2. Heintzman, H. D., Stuart, R. K., Hon, G., Fu, Y., Ching, C. W., Hawkins, R. D., Barrera, L. O., Van Calcar, S., Qu, C., Ching, K. A., Wang, W., Weng, Z., Green, R. D., Crawford, G. E., Ren, B. Distinct and predictive chromatin signatures of transcriptional promoters and enhancers in the human genome. Nature Genet. 39: 311-318, 2007.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

62780 MW

Observed Molecular Weight

200 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Mouse, Rat<br>

Subcellular Location

Apical cell membrane ; Multi-pass membrane protein . Colocalizes with PDZK1 on apical membranes of kidney proximal tubules.

Protein Name

Solute carrier family 22 member 5