Anti-Progesterone Receptor/PGR Antibody Picoband®, iFluor647 Conjugate

About this product

Boster Bio Anti-Progesterone Receptor/PGR Antibody Picoband® catalog # PB9009. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9009-iFluor647

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The progesterone receptor (PR) is an intracellular steroid receptor that specially binds progesterone in humans. PR has been a member of the steroid receptor superfamily. It is encoded by a single PGR gene residing on chromosome 11q22. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. It can be observed in human breast tissues. The proteins function as dimeric molecules in nuclei to regulate the transcription of target genes in a ligand-responsive manner.

Synonyms

Progesterone receptor;PR;Nuclear receptor subfamily 3 group C member 3;PGR;NR3C3;

Gene Name

Progesterone receptor

UniProt

P06401

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human Progesterone Receptor recombinant protein (Position: M595-K933). Human Progesterone Receptor shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Progesterone Receptor, respectively.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine. .

Applications

IF,IHC,ICC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Misrahi M, Atger M, d'Auriol L, Loosfelt H, Meriel C, Fridlansky F, Guiochon-Mantel A, Galibert F, Milgrom E (March 1987). "Complete amino acid sequence of the human progesterone receptor deduced from cloned cDNA". Biochem. Biophys. Res. Commun. 143 (2): 740–8. 2. Law ML, Kao FT, Wei Q, Hartz JA, Greene GL, Zarucki-Schulz T, Conneely OM, Jones C, Puck TT, O'Malley BW (May 1987). "The progesterone receptor gene maps to human chromosome band 11q13, the site of the mammary oncogene int-2". Proc. Natl. Acad. Sci. U.S.A. 84 (9): 2877–81. doi:10.1073/pnas.84.9.2877. PMC 304763. PMID 3472240. 3. Nishihara T, Kitaura M, Imagawa M, Nishikawa J (1995). "Vitamin D receptor contains multiple dimerization interfaces that are functionally different". Nucleic Acids Res. 23 (4): 606–611. 4. Schmitt J, De Vos P, Verhoeven G, Stunnenberg HG (1994). "Human androgen receptor expressed in HeLa cells activates transcription in vitro". Nucleic Acids Res. 22 (7): 1161–1166.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

98981 MW

Observed Molecular Weight

90 kDa (PR-A), 118 kDa (PR-B)

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Human<br> Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat<br> Immunocytochemistry/Immunofluorescence, 5μg/ml, Human<br>

Subcellular Location

Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G (1) and G (2)/M phases.

Sequence Similarities

Belongs to the nuclear hormone receptor family. NR3 subfamily.

Protein Name

Progesterone receptor