Anti-Eotaxin/CCL11 Antibody Picoband®, iFluor647 Conjugate

About this product

Boster Bio Anti-Eotaxin/CCL11 Antibody Picoband® catalog # PB9065. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

PB9065-iFluor647

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

CCL11, also known as Eotaxin, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. CCL11 is a small cytokine belonging to the CC chemokine family. It is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The effects of CCL11 are mediated by its binding to a G-protein-linked receptor known as a chemokine receptor.

Synonyms

Eotaxin;C-C motif chemokine 11;Eosinophil chemotactic protein;Small-inducible cytokine A11;Ccl11;Scya11;

Gene Name

Eotaxin

Gene ID

20292

UniProt

P48298

Host

Rabbit

Reactivity

Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived mouse Eotaxin recombinant protein (Position: H24-P97). Mouse Eotaxin shares 62% and 97% amino acid (aa) sequences identity with human and rat Eotaxin, respectively.

Clonality

Polyclonal

Tissue Specificity

Expressed constitutively in the thymus. Expression inducible in the lung (type I alveolar epithelial cells), intestine, heart, spleen, kidney.

Applications

WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

In response to the presence of allergens, this protein ly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils.

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

References & Citations

1. Ogilvie, P.; Bardi, G.; Clark-Lewis, I.; Baggiolini, M.; Uguccioni, M. Eotaxin is a natural antagonist for CCR2 and an agonist for CCR5. Blood 97: 1920-1924, 2001. 2. Hein, H.; Schluter, C.; Kulke, R.; Christophers, E.; Schroder, J.-M.; Bartels, J. Genomic organization, sequence, and transcriptional regulation of the human eotaxin gene. Biochem. Biophys. Res. Commun. 237: 537-542, 1997.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

10893 MW

Observed Molecular Weight

11 kDa

Fragment

Rabbit IgG

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.1-0.5μg/ml, Mouse<br>

Subcellular Location

Secreted.

Sequence Similarities

Belongs to the Wnt family.

Protein Name

Eotaxin