Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody Picoband® (Carrier-free Conjugate)

About this product

Boster Bio Anti-Protein Kinase A regulatory subunit I alpha/PRKAR1A Antibody Picoband® catalog # A00699-1. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Specifications

Catalog number

A00699-1-carrier-free

Size / count

100 µg/Vial

Dry ice

No

Hazardous

No

Discontinued

No

Background

cAMP-dependent protein kinase type I-alpha regulatory subunit is an enzyme that in humans is encoded by the PRKAR1A gene. This protein encoded by this gene was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Several alternatively spliced transcript variants encoding two different isoforms have been observed.

Synonyms

cAMP-dependent protein kinase type I-alpha regulatory subunit;Tissue-specific extinguisher 1;TSE1;cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed;PRKAR1A;PKR1, PRKAR1, TSE1;

Gene Name

cAMP-dependent protein kinase type I-alpha regulatory subunit

UniProt

P10644

Host

Rabbit

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PRKAR1A recombinant protein (Position: E2-E81). Human PRKAR1A shares 89.9% and 92.4% amino acid (aa) sequence identity with mouse and rat PRKAR1A, respectively.

Clonality

Polyclonal

Tissue Specificity

Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Applications

IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. .

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Applications Notes

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat <br>Western blot, 0.1-0.5μg/ml, Human, Rat<br>

Subcellular Location

Cell membrane .

Protein Name

cAMP-dependent protein kinase type I-alpha regulatory subunit