Anti-Actin ACTA1 Antibody (Monoclonal, AC-40), Carrier-free

About this product

Boster Bio Anti-Actin ACTA1 Antibody (Monoclonal, AC-40) catalog # MA1000. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Specifications

Catalog number

MA1000-carrier-free

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin, two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins, this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.

Synonyms

Actin, alpha skeletal muscle;Alpha-actin-1;ACTA1;ACTA;

Gene Name

Actin, alpha skeletal muscle

Gene ID

Human(58), Mouse(11459), Rat(29437), Human(70), Mouse(11464), Rat(29275), Human(59), Mouse(11475), Rat(81633), Human(60), Mouse(11461), Rat(81822), Human(71), Mouse(11465), Rat(287876), Human(72), Mouse(11468), Rat(25365)

UniProt

P68133

Host

Mouse

Reactivity

Chicken,Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Synthetic actin C-terminal peptideSer-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe,attached to a Multiple Antigen Peptide (MAP) backbone.

Clonality

Monoclonal

Clone

Clone: AC-40

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Applications

IHC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Gunning,P., Ponte,P., Okayama,H., Engel,J., Blau,H. and Kedes,L.Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. Mol. Cell. Biol.1983; 3 (5), 787-795. 2. Goebel,H.H., Brockmann,K., Bonnemann,C.G., Warlo,I.A., Hanefeld,F.,Labeit,S., Durling,H.J. and Laing,N.G.Actin-related myopathy without any missense mutation in the ACTA1 Gene. J. Child Neurol.2004; 19 (2), 149-153. 3. Laing,N.G., Clarke,N.F., Dye,D.E., Liyanage,K., Walker,K.R.,Kobayashi,Y., Shimakawa,S., Hagiwara,T., Ouvrier,R., Sparrow,J.C., Nishino,I., North,K.N. and Nonaka,I.Actin mutations are one cause of congenital fibre type disproportion.Ann. Neurol.2004; 56 (5), 689-694.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

42051 MW

Observed Molecular Weight

43 kDa

Fragment

Mouse IgG2a

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Frozen Section), 4μg/ml, Human, chicken, mouse, rat, -<br>Western blot, 2μg/ml, Human, chicken, mouse, rat<br>

Subcellular Location

Cytoplasm, cytoskeleton.

Protein Name

Actin, alpha skeletal muscle|Actin, alpha cardiac muscle 1|Actin, aortic smooth muscle|Actin, cytoplasmic 1|Actin, cytoplasmic 2|Actin, gamma-enteric smooth muscle