Anti-CD8 Cd8A Antibody (Monoclonal, CA-8), Carrier-free

About this product

Boster Bio Anti-CD8 Cd8A Antibody (Monoclonal, CA-8) catalog # MA1017. Tested in Flow Cytometry, IHC, ICC applications. This antibody reacts with Human.

Specifications

Catalog number

MA1017-carrier-free

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

The human lymphocyte differentiation antigen CD8 is encoded by a single gene that gives rise to a 33- to 34-kDa glycoprotein expressed on the cell surface as a dimer and in higher molecular mass forms.

Synonyms

T-cell surface glycoprotein CD8 beta chain;CD8 antigen 37 kDa chain;OX-8 membrane antigen;CD8b;Cd8b;Cd8b1;

Gene Name

CD8A; CD8B

Gene ID

Human(925), Mouse(12525), Rat(24930)

UniProt

P01732

Host

Mouse

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Human thymocytes followed by peripheral blood T cells.

Clonality

Monoclonal

Clone

Clone: CA-8

Tissue Specificity

Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. .

Applications

Flow Cytometry,IHC,ICC

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Giblin, P.; Ledbetter, J. A.; Kavathas, P. : A secreted form of the human lymphocyte cell surface molecule CD8 arises from alternative splicing. Proc. Nat. Acad. Sci. 86: 998-1002, 1989.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

23400 MW

Observed Molecular Weight

25 kDa

Fragment

Mouse IgG2a

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Frozen Section), 1μg/ml, Human<br>Immunocytochemistry, 0.5-1μg/ml, Human<br> Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.