Anti-Collagen, Type III Col3a1 Antibody (Monoclonal, FH-7A), Carrier-free

About this product

Boster Bio <a href="https://www.bosterbio.com/anti-collagen-i-antibody-pa2140-1-boster.html" style="color:#EA8D28">Anti-Collagen</a>, Type III Col3a1 Antibody (Monoclonal, FH-7A) catalog # MA1029. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Specifications

Catalog number

MA1029-carrier-free

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Collagen type III, also known as COL3A1, is assigned to chromosome 2. Ultrastructural analysis of tissues from mutant mice revealed that type III collagen is essential for normal collagen I fibrillogenesis in the cardiovascular system and other organs. Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development.

Synonyms

Collagen alpha-1 (III) chain;Col3a1;

Gene Name

Collagen alpha-1(III) chain

Gene ID

Human(1281), Mouse(12825), Rat(84032)

UniProt

P13941

Host

Mouse

Reactivity

Human,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Human collagen type III.

Clonality

Monoclonal

Clone

Clone: FH-7A

Tissue Specificity

Expressed on the plasma membrane of hepatocytes and in the narrow apical portions of supporting cells in the vomeronasal sensory epithelium. Detected in the type III alveolar cells of the lung, in the proliferative crypt epithelium of the small intestine and in the older intragemmal cells of the tongue. .

Applications

IHC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12 (By similarity). .

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Cutting, G. R.; McGinniss, M. J.; Kasch, I. M.; Tsipouras, P.; Antonarakis, S. E. : Physical mapping by PFGE localizes the COL3A1 and COL5A2 genes to a 35 kb region on chromosome 2. (Abstract) Clin. Res. 38: 266A, 1990. 2. Emanuel, B. S.; Cannizzaro, L. A.; Seyer, J. M.; Myers, J. C. : Human alpha-1(III) and alpha-2(V) procollagen genes are located on the long arm of chromosome 2. Proc. Nat. Acad. Sci. 82: 3385-3389, 1985. 3. Liu, X.; Wu, H.; Byrne, M.; Krane, S.; Jaenisch, R. : Type III collagen is crucial for collagen I fibrillogenesis and for normal cardiovascular development. Proc. Nat. Acad. Sci. 94: 1852-1856, 1997.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

138936 MW

Observed Molecular Weight

49 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, rat, -<br>Western blot, 0.25-0.5μg/ml, Human, rat<br>

Subcellular Location

Secreted, extracellular space, extracellular matrix .

Sequence Similarities

Belongs to the fibrillar collagen family.

Protein Name

Collagen alpha-1(III) chain