Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26), Carrier-free

About this product

Boster Bio Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26) catalog # MA1082. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

MA1082-carrier-free

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.

Synonyms

Keratin, type II cytoskeletal 8;Cytokeratin endo A;Cytokeratin-8;CK-8;Keratin-8;K8;Type-II keratin Kb8;Krt8;Krt2-8;

Gene Name

Keratin, type II cytoskeletal 8

UniProt

Q10758

Host

Mouse

Reactivity

Human,Mouse

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Cytokeratin from human epidermis.

Clonality

Monoclonal

Clone

Clone: PCK-26

Tissue Specificity

Expressed in cardiac and striated muscle. Expressed at Z-lines within the muscle fibers and at Z-line and M- line domains at costameres at the sarcolemmal membrane (at protein level). Observed in coagulating gland, bladder, salivary gland, kidney, spleen, thymus, lung and heart. Also observed in ventral prostate, seminal vesicle and liver where expression increases following castration. .

Applications

IF,IHC,WB

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. .

Components

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

References & Citations

1. Rastaldi MP et al. Glomerular podocytes contain neuron-like functional synaptic vesicles. FASEB J 20:976-8(2006). 2. Wen J et al. Use of superparamagnetic microbeads in tracking subretinal injections. Mol Vis 11:256-62 (2005).

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

54019 MW

Observed Molecular Weight

54 kDa

Fragment

Mouse IgG1

Specificity

No cross reactivity with other proteins.

Applications Notes

Western blot, 0.5-1μg/ml, Human, mouse<br> Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, By Heat <br> Immunofluorescence, 1-2μg/ml, Human, -<br>

Subcellular Location

Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .

Sequence Similarities

Belongs to the intermediate filament family.

Protein Name

Keratin, type II cytoskeletal 5